Laishram Shantikumar Singh: Profile

Laishram Shantikumar Singh Photo

Laishram Shantikumar Singh

Laishram Shantikumar Singh, PhD
Designation/Position: Scientist-B
Department/Programme Name: Microbial Resources Programme
Phone: 03592-231703; Extension No.: 212


Academic Qualifications:
•    2003 - 2008, Ph. D, Dibrugarh University:  Research work carried out at CSIR-NEIST, Jorhat, Assam.
•    1999 - 2001, M.Sc. Microbiology, Bangalore University, Bengaluru. 

Experience:
•    2016 - Present, Scientist B, IBSD Sikkim Centre, Gangtok, Sikkim.
•    2011 - 2016, Scientist B, IBSD Sikkim Centre, Gangtok, Sikkim.
•    2009 - 2011: Assistant Manager R & D and Microbiologist, Research and Development Centre, APPL (Tata Tea Research), Teok, Jorhat, Assam.
•    2008 - 2009: Post Doctoral Research Scholar, University School of Biotechnology, Guru Gobind Singh Indraprastha University, New Delhi.
•    2002 - 2007: JRF and SRF, DRL, DRDO, Tezpur, Assam. (Ph.D. Research work under CSIR- NEIST, Jorhat, Assam).
•    2001 - 2002: Lecturer, Brindavan College of UG & PG Sciences, Bangalore University, Bengaluru.

Awards & Fellowships:
•    2009: “Group Technology Award 2009” given by DRL, DRDO, Ministry of Defence, Govt. of India, Tezpur, Assam, for the formulation and evaluation of herbal product “Herbol”, a Germicidal Floor Cleansing Emulsifier.
•    2002-2007: JRF and SRF awarded by Defence Research and Development Organisation (DRDO), Ministry of Defence, Govt. of India, New Delhi.
•    2001: “Academic Excellence Award” by Post Graduate Department of Microbiology, NFG College, Bangalore University, Bengaluru.
•    1992: 46th Rank in state level “First Search Talent Examination in Science” awarded by Besant Academy, Sastri Nagar, Chennai, Tamil Nadu.
•    1990: 2nd position in state level Quiz competition on the occasion of National Science Day awarded by Science Teachers’ Forum, Manipur.
•    2018: Best Poster Award for the poster entitled “Bioprospecting of Endophytes of Important Medicinal Plants viz. Panax spp. of Sikkim Himalayas for their bioactive compounds: A Chemical Ecology Prospect” by Singh, L. S., Shankaar, R. U., Rai, S. during the 3rd Annual SAC & PSC meeting of the DBT funded Project - Chemical Ecology of the North East Region (NER) of India: A Collaborative Programme Linking NER and Bangalore Researchers held at Imphal from 19 - 20th April 2018.

Memberships (Professional Associations / Societies)

•    Annual member of Indian Science Congress Association

Journal/Academic Editors
•    Managing Editor of special issue of “Encyclopaedia of Bioscience”, Frontiers in Bioscience
•    Editorial Board Member, Cell and Cellular Kinetics journal, Oscine Publications
•    Editorial Board Member, Acta Scientific Microbiology, Acta scientific Open Access
•    Editorial Board Member, Archives of immunology and allergy, SRYAHWA Publications

Specialized Training

•    2002: Proficiency course in “Bioprocess Engineering” held at IISc. Bangalore, Bengaluru.  
•    2003: Mass production of bacterial strain in 100 L Fermentor, Recovery & bio-efficacy testing of the cell mass against mosquito larvae at Vector Control Research Centre, ICMR, Puducherry.
•    2003: CEP Course on “Recent trends in Malaria and its control” held at DRL, DRDO, Tezpur, Assam.
•    2004: CEP Course on “Application of Remote sensing and GIS in the management of Malaria” held at DRL, DRDO, Tezpur, Assam.
•    2004: CEP Course on “Application of Botanicals in the management of pathogenic fungi” held at DRL, DRDO, Tezpur, Assam. 
•    2004: CEP Course on “Naturally Occurring Microbes for Control of Human Disease” held at DRL, DRDO, Tezpur, Assam.
•    2006: CEP Course on “Molecular Approaches in Disease Diagnosis” held at DRL, DRDO, Tezpur, Assam. 

Other Achievements
•    Product Developed: Core team member in the formulation and development of a herbal product, “ Herbal Floor Cleaner ” a Germicidal Floor Cleansing Emulsifier developed by DRL, DRDO Tezpur, Assam and TOT given to M/s Biotech International Ltd., B-21, Site C, Surajpur, Greater Noida, Uttar Pradesh – 201306 by DRL in 2009.

1.    Research theme photo

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2.    Verticals involved
•    Microbial Resources
•    Ethnobotany, Ethnopharmacology & Drug Discovery

3. Research Area/Research Expertise
•    Microbial Biotechnology.
•    Bioprospecting for microbial (Actinomycetes, Endophytes) secondary metabolites. 
•    Phytochemicals against dermatophytes, mitigate drug resistant & opportunistic pathogens. 
•    Biocontrol Agents, Plant Endophyte Relation, Chemical Ecology of Endophytes.
•    Endophytes from medicinal plants & their host secondary metabolites.

4. Research Summary
    A. Current Research:
•    Exploration and Bioprospecting of Microbial Flora of the Sikkim Himalayas for their Possible Application in Agriculture and Therapeutics:
The ongoing research work mainly focused on exploration and bioprospecting of indigenous microbial strains from the pristine habitats of Sikkim Himalayas for their potentiality towards the development of biocontrol agent to mitigate the burden of plant diseases and also to search for a promising antimicrobial agent for therapeutic applications.

•    Screening and characterization of bioactive metabolites from Actinomycetes of Sikkim Himalayas for antimicrobial agent:
The rapid emergence and dissemination of drug resistance among microbial pathogens including the opportunistic pathogens has mounted pressure and compounded the need for the search of effective, safer and novel antimicrobials to mitigate the burden of drug resistance. Hence actinomycetes, the prolific producer of bioactive metabolites, antibiotics and other compounds would be investigated. The Sikkim Himalayan region being a part of the Biodiversity hot spots has enormous potential for exploring the bioactive metabolites from untapped indigenous actinomycetes flora. Hence tremendous opportunities in bioprospecting for novel molecules out of these unique pristine habitats cannot be rule out. It’s imperative to highlight the proposition of “Renaissance in antibacterial discovery from actinomycetes”. In our view, making the uncultured from untapped habitat to pure culture is one new hope for getting new drug leads which would offer great potential to mitigate ever increasing burden of drug resistance in health care sector. Our group mainly focuses on bioprospecting of actinomycetes & rare actinomycetes from unique pristine ecological niches for their therapeutic potential towards sustainable utilization of the untapped microbial resources for the benefit of mankind.

•    Isolation, characterisation and evaluation of indigenous microbial flora of Sikkim Himalayas for potential utilization as biocontrol agent
Phytopathogens pose serious problems in the cultivation of economically important plants. Chemical pesticides cause several harmful effects to the environment as well as to the human health at large. An alternative to chemical pesticides is the Biocontrol agent which is more environments friendly, involves the use of naturally occurring non pathogenic microorganism that set to reduce the activity of plant pathogens and thereby suppress diseases. Antagonistic microorganism can compete with the pathogen for nutrients, inhibit pathogen multiplication by secreting antibiotics or toxins or reduce pathogen population through hyperparasitisms. The Sikkim Himalayan region being a part of the Biodiversity hot spots has enormous potential for exploration and bioprospecting of the untapped indigenous microflora which could be exploited for biocontrol applications. The main focus of the ongoing research work is bioprospecting of indigenous microbial strains from the Sikkim Himalayas for their potentiality towards the development of biocontrol agents for organic farming practice for sustainable agriculture. Biological control approach could be one of the promising strategies to combat the aggravating problem of plant disease.

•    Bioprospecting of Endophytes of Medicinal Plants viz. Panax spp. of Sikkim Himalayas for their bioactive compounds: A Chemical Ecology Prospect:
Endophytes are microbes that reside in the plant tissues without causing any apparent harm to the host plant. They form inconspicuous infections within tissues of healthy plants for all or nearly all their life cycle. Endophytes which are ubiquitous in all plant species in the world, contribute to their host plants by producing variety of compounds/substances that provide protection and thereby survival value to the plant. It is opined that endophytes are new and potential source of novel natural products for exploitation in modern medicine, agriculture and industry. To date many novel natural products possessing antimicrobial activities and other bioactive metabolites have been isolated from endophytes. It is worth mentioning that screening for antimicrobial compounds and other functionalities from endophytes is a promising way to overcome the increasing threat of drug resistant strains of human and plant pathogen. Our group also focussed on the potential of endophytes from indigenous traditional medicinal plants of high altitude region for production of host plant secondary metabolites. Exploration of associated endophytes and screening for their capacity to yield host plant secondary metabolites as well as other compounds is a promising way for producing the bioactive compounds and also holds to protect the plant species. We are interested in studying the plant endophyte relation and their cross talk due to the co-existence and evolution. The ability of the endophytes to produce the same or similar bioactive substances as those originated from the host plants would be beneficial to study the relations between the endophytes and their host plants. It would enable to develop a substitutable approach for efficiently producing these scarce and valuable bioactive compounds. It intends to reduce the need to harvest slow-growing and rare medicinal plants as well as preserve the world’s ever-diminishing biodiversity and save the delicate ecosystem.

 
B. Previous Notable Research:
•    Mass production of Indigenous Entomopathogenic soil bacterial strain, biomass recovery & bio-efficacy testing of the cell mass against mosquito larvae, carried out at Defence Research Laboratory, DRDO, Tezpur- 784001, Assam.
The larvicidal activity of natural isolates of indigenous entomopathogenic soil bacteria from different mosquito breeding habitats of Sonitpur district of Assam, India was carried out and the potential isolate, Bacillus sphaericus GC sub group IV was selected and its mass production of the cell biomass was carried out using fermentor. Bioassay of the lyophilized biomass of the selected isolate was carried out against laboratory reared Cx. quinquefasciatus larvae. The bioassay of the potent isolate was also carried out against An. stephensi and Ae. albopictus. The bio-efficacy of the cell mass was carried out against mosquito larvae in comparison with some commercial biolarvicides. The efficacy of B. sphaericus GC sub group IV against Cx. quinquefasciatus was found to be more when compared with that of commercial larvicides such as Spherix (B. sphaericus) and ABG 6262 (B. sphaericus), which highlights the potential of this strain.

•    Exploration of medicinal plants (essential oil, plant extracts) for antimicrobial property, carried out at Defence Research Laboratory, DRDO, Tezpur- 784001, Assam.
Methanolic and ethanolic extracts of various plants from Sonitpur district of Assam was evaluated for antimicrobial potentiality against five common and clinically significant bacterial pathogens viz. Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa and Shigella flexneri. S. aureus was found to be the most sensitive while K. pneumoniae was the least sensitive. The ethanolic extract of Piper thomsoni exhibits the maximum inhibition against S. aureus when compare to Ciprofloxacin 10 mg. Both methanol and ethanol extracts of Garcinia pedunculata could inhibit all the tested bacteria except K. pneumoniae. Amongst the tested bacterial strains K. pneumoniae was the most resistant bacteria followed by P. aeruginosa, while S. aureus was the most sensitive. The antibacterial potential of the essential oil from rhizome of Homalomena aromatica was evaluated against five ubiquitous strains of both Gram-positive & Gram-negative bacterial pathogens. S. aureus was found to be the most sensitive while P. aeruginosa was the least sensitive among the tested pathogens. It was observed that the essential oil of H. aromatic displayed more inhibitory effect against Gram-positive pathogen when compared to Gram-negative pathogens. The in-vitro activity of the oil was compared with that of Gentamicin.

•    Development of the formulation of Herbal Germicidal Floor Cleansing Emulsifier, “Herbol” carried out at Defence Research Laboratory, DRDO, Tezpur- 784001, Assam
General disinfectants available in the market contain harmful chemicals such as formaldehyde, n-propanol, benzalkonium chloride, phenol etc. To get rid of these, development of disinfectants that are non toxic and bio-safe is highly invited. I was actively involved and core member in the development of a germicidal herbal formulation developed by Defence Research Laboratory, Tezpur, Assam. The Floor Cleansing Herbal Germicidal Liquid formulation comprised of essential oils from two local plants along with other ingredients. This herbal formulation finds excellent application in schools, hospitals, restaurants and office buildings for cleaning floors and tiles. A single application removes dirt and grime. It also inhibits the bacterial growth where odours are problem. This germicidal herbal formulation gives a lasting sweet fragrance for 3 to 4 hours. It is Non-toxic, eco-friendly and gives pleasant odour, Acts as fungicide and bactericide, Cost effective. TOT of the product has been given to M/s Biotech International Ltd., B-21, Site C, Surajpur, Greater Noida, Uttar Pradesh - 201306.

•    Exploration of microorganism from North East India for their potential applications in agriculture and Pharmaceutical sector, carried out at CSIR-NEIST Jorhat, Assam.
Due to their functional diversity, microorganisms have found applications in agriculture, industry, medicine and environment. Isolation, characterisation and screening of several microbial strains from different biotope of NE region of India was carried out. Accomplished in screening good number of microbial metabolites from fresh water lake as well as forest area against targeted pathogens and their complete bioassay. Isolation and bioassay guided purification of bioactive metabolite from selected potential isolate was carried out. A broad-spectrum antibiotic producing alkaliphilic actinomycetes isolate A2D from phoomdi in Loktak Lake of Manipur, India has been characterised and identified as Streptomyces tanashiensis strain A2D. Optimisation of nutritional factors for antibiotic production by the strain was studied and found that the antibiotic production was positively affected by the carbohydrates, nitrogen sources and NaCl. The strain A2D showed a broad antimicrobial spectrum against Gram-positive bacteria, Gram-negative bacteria and fungi. The active metabolite exhibited lowest MIC against Staphylococcus aureus whereas highest MIC was recorded against Candida albicans and Fusarium moniliform. So far only few alkaliphilic actinomycetes have been explored for their antimicrobial potential.

•    Timber wood protection from wood decaying basidiomycetes using actinomycetes, carried out at University School of Biotechnology, Guru Gobind Singh Indraprastha University, New Delhi.
Worked as post doctoral scholar in a Project entitled “Timber wood protection from wood decaying basidiomycetes using actinomycetes” funded by Ministry of Environment and Forestry at USBT, GGSIP University. Wood-rotting fungi colonize and degrade the wood using enzymatic and non-enzymatic processes. Decay and discoloration caused by fungi are major sources of loss in both timber production and wood use. Actinomycetes being the prolific and potent producer of bioactive metabolites, enzymes and other functionalities were screened for antagonistic activity against wood decaying fungi. Several strains were evaluated. Actinomycetes isolate from Loktak Lake soil, of Manipur, which has been characterised and identified as Streptomyces sp. MT7 exhibited strong and broad spectrum antagonism towards seven out of eight tested wood-rotting fungi. Strain MT7 secretes three vital fungal cell wall lytic enzymes, i.e. chitinase, ?-1,3- glucanase, and protease, and siderophores. Extracellular fluid (ECF) and its organic solvent extract also exhibited potential antagonism towards the tested wood-rotting fungi. Antifungal metabolites were characterized as polyene in nature. Biocontrol traits like co-production of cell wall lytic enzymes and antifungal secondary metabolites including siderophores by Streptomyces sp. MT7 highlights its importance & suggests that it could be employed as a potential biocontrol agent against wood-rotting basidiomycetes.

•    Development and formulation of Biofertilizers and Biocontrol Agents for organic cultivation of tea plants, carried out at Research and Development Centre, APPL, (Former Tata Tea Research) Teok, Jorhat, Assam.
Owing to the concerns and undesirable effects of chemicals and pesticides, there has been demand as well as need for organic cultivation of tea. Organic tea has its charm and demand. Several indigenous microbial strains of Phosphate Solubilising Bacteria (PSB) as well as Nitrogen fixing bacteria, Azospirillum were isolated from local tea garden soils. They were evaluated for the functional traits of Phosphate solubilising potential and nitrogen fixing ability respectively. The potential PSB strain (Pseudomonas) and Azospirillum were selected and mass production was carried out for application in the tea garden in place of chemical fertilizers. In this initiative several tea gardens under APPL plantations in Assam have undertaken the application of microbial inputs as Biofertilizers towards the organic cultivation of tea. Similarly Trichoderma strains were formulated and mass production was carried out for application in tea gardens for the control of tea plant pathogens.

•    Screening of microbial flora of Phoomdi of Loktak Lake of Manipur for antimicrobial metabolites, carried out at IBSD, Sikkim   
   
Actinomycetes have provided important bioactive compounds of high commercial value and continue to be routinely screened for new bioactive substances. About 45% of the presently known bioactive microbial metabolites were isolated from various actinomycetales species. Screening of actinomycetes from Phoomdi soil of Loktak Lake of Manipur, India has led to the characterisation and identification of Streptomyces sannanensis SU118 which showed antimicrobial activity only against Gram-positive bacteria, while Gram-negative organisms were not affected. The antimicrobial agent produced by the strain exhibited lowest MIC against S. aureus MTCC 96 and S. aureus (clinical isolate), whereas highest was recorded against Mycobacterium smegmatis MTCC 6 and Bacillus circulans MTCC 8074. This highlights that detection and identification of members of the genus Streptomyces are of great value because they provide a rich source of antibiotics.

A.    Research Articles (Peer Reviewed, Only authorized publishers)
1.    Singh L S, Sharma H, Sahoo D (2019) Actinomycetes from Soil of Lachung, a Pristine High Altitude Region of Sikkim Himalaya, Their Antimicrobial Potentiality and Production of Industrially Important Enzymes. Advances in Microbiology. 9(8):750-773, Impact Factor 1.30, https://file.scirp.org/pdf/AiM_2019081515121534.pdf

2.    Singh L S, Sharma H, Sahoo D (2019) Actinomycetes: the Unparallel Potential Source of Antibiotics and Natural Products for Drug Discovery. Acta Scientific Microbiology. 2(9):15-17, Impact factor 1.125. https://actascientific.com/ASMI/pdf/ASMI-02-0324.pdf

3.    Tiwari A, Sherpa YL, Pathak A P, Singh LS, Gupta A, Tripathy A (2019) One-pot green synthesis of highly luminescent silicon nanoparticles using Citrus limon (L.) and their applications in luminescent cell imaging and antimicrobial efficacy. Materials Today Communications. 19: 62-67, Impact factor 1.859, https://doi.org/10.1016/j.mtcomm.2018.12.005

4.    Deori DJ, Abujam S, Dakua S, Laishram  S (2017) Investigation on Feeding, Growth and Condition Factor of Certain Ornamental Fish From Brahmaputra River at Dibrugarh, Assam, India.  Journal of FisheriesSciences.com. 11(3): 043-047, Impact factor 1.40,
     www.fisheriessciences.com/archive/ipfs-volume-11-issue-3.
    
5.    Singh L S, Sharma H, Talukdar NC (2014) Production of potent antibiotic by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak Lake of Manipur, India. BMC Microbiology. 14:278, Impact factor 3.372. PubMedID: 25406714.

6.    Nagpure A, Choudhary B, Shantikumar, Gupta RK (2014). Isolation and characterization of chitinolytic Streptomyces sp. MT7 and its antagonism towards wood-rotting fungi. Annals of Microbiology.  64 (2): 531-541, Impact  factor 1.596, https://link.springer.com/article/10.1007%2Fs13213-013-0686-x

7.    Singh L S, Mazumder S, Bora TC (2009) Optimisation of process parameters for growth and bioactive metabolite produced by salt-tolerant and alkaliphilic actinomycetes - Streptomyces tanashiensis strain A2D. Journal De Mycologie Medicale. 19: 225-233, Impact factor 1.661, https://www.em-consulte.com/en/article/235906

8.    Laishram SKS, Das NG, Bailung B, Dube SN, Srivastava RB (2008) Evaluation of some medicinal plants of North-Eastern India for antibacterial activity: A preliminary study. Journal  of cell and Tissue Research.  8(1): 1285-1287, https://tcrjournals.com/tr_pastabstract.php?vid=27

9.    Baruah I, Laishram SKS, Das SC, Kalita J (2008) Larvicidal activity of indigenous Entomopathogenic soil bacteria from Northeastern region of India. Journal of cell and Tissue Research. 8 (1): 1271-1274. https://tcrjournals.com/tr_pastabstract.php?vid=27

10.    Singh LS, Baruah I, Bora TC (2006) Actinomycetes of Loktak Habitat: Isolation & Screening for Antibacterial Activities. Biotechnology. 5 (2): 217-221. https://scialert.net/abstract/?doi=biotech.2006.217.221

11.    Singh L S, Bailung B, Baruah I, Bora TC (2006) Evaluation of antimicrobial activity of Bacteria isolated from Loktak Lake of Manipur, India. Asian Journal of Microbiology Biotechnology & Environmental Science.  8 (2):  271-273, http://www.envirobiotechjournals.com/article_abstract.php?aid=1562&iid=61&jid=1

12.    Singh LS, Bora TC (2006) Antifungal spectra of activity of Streptomyces S 124 strain against plant pathogenic fungi. Indian phytopathology. 59 (3): 400.

13.    Laishram SKS, Nath DR, Bailung B, Baruah I (2006) In vitro antibacterial activity of essential oil from rhizome of Homalomena aromatica against pathogenic bacteria. Journal  of cell and Tissue Research.  6(2): 849-851, https://tcrjournals.com/tr_pastabstract.php?vid=24

14.    Goswami S, Singh L S (2006) Antibacterial Potential of Some Indigenous plants Against Human Pathogenic Bacteria. Environment and Ecology. 24S (2): 403- 405.

B.    Technology Transferred
•    Nath DR, Gogoi BJ, Goswami S, Singh L S, Srivastava RB, (2009) Herbal Floor Cleaner, [Ref. MOU for TOT for Herbal Floor Cleaner- 1390/10/06/2009], to M/S. Biotech International Ltd. Greater Noida, UP.

C.    Patents
•    Nath DR, Gogoi BJ, Goswami S, Singh L S, Srivastava RB. (30/04/2007) Germicidal Floor Cleansing Emulsifier. (Application No. 286/DEL/2007), DRDO, New Delhi, Status: Filed. 

D.    Book Chapters
1.    Mazumder S, Singh L S, Bora TC (2012) Endophytic fungi associated with medicinal plants of Gibbon Wild Life Sanctuary, India and their antagonistic properties. Advances in Life Sciences: Principles and Applications. Studium press LLC, Houston, USA, PP: 213 - 224. ISBN: 1-933699-69-8.

Group Photo: 06102020220POZB3QuLRX3l.jpg

Group Members (Details):

1.

Miss Subecha Rai, JRF,                  (DBT Funded Project)

M.Sc. Microbiology, GATE & ARS NET qualified.

Email: subegaara@gmail.com

Project Title : “ Chemical Ecology of the North East Region (NER) of India: A Collaborative Programme Linking NER and Bangalore Researchers”

Problem Addressing : “ Bioprospecting of Endophytes of Important Medicinal Plants viz. Panax spp. of Sikkim Himalayas for their bioactive compounds: A Chemical Ecology Prospect”  

Expected Outcome : Endophytes from the Panax plant could produce host plant secondary metabolite in in-vitro condition.

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2.

Miss Varsha Thapa, JRF,             (DBT funded Project)

M. Sc. Biotechnology, M.Phil.

Email: varshathapa90@gmail.com

Project Title : “ Chemical Ecology of the North East Region (NER) of India: A Collaborative Programme Linking NER and Bangalore Researchers”

Problem Addressing : “Evaluation of Endophytes of native plants of Sikkim Himalaya viz . Paris polyphylla Smith and Amomum subulatum Roxb . for potential biological control of phytopathogenic fungi”.

Expected Outcome : Endophytes from the target plant could exhibit potential antagonistic effect towards viable biocontrol agents.

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3.

Mrs. Sita Devi Sharma, Project Assistant ,           (DBT Project)

E mail: sitasharma833@gmail.com

Project Title : “ Chemical Ecology of the North East Region (NER) of India: A Collaborative Programme Linking NER and Bangalore Researchers”

Problem Addressing: To assist in the field and laboratory activities of the project work.

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4

Miss Annu Khati,      Project Assistant ,               (DBT project)

Email:   annukhati1@gmail.com

Project Title : “ Chemical Ecology of the North East Region (NER) of India: A Collaborative Programme Linking NER and Bangalore Researchers”

Problem Addressing: To assist in the field and laboratory activities of the project work.

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5

Miss Rabina Gurung,    Project Fellow,                (IBSD Core)

M. Sc. Botany

Email: rabinagurung74@gmail.com

Problem Addressing : Isolation and evaluation of indigenous microbial flora of Sikkim Himalayas for potential utilization as biocontrol agent.

Expected Outcome : Indigenous actinomycetes isolates from Sikkim region could be envisage for effective biological control agent.

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Previous Members (Alumni)

1.

Mrs. Radha Kumari Basnett, STA, IBSD Core       (2011-2012).

M.Sc. Microbiology.

Worked on : Isolation of microorganism from soil, pure culture maintenance and other laboratory activities.

Currently working as : Technical Assistant, Department of Microbiology, Sikkim University, Gangtok, Sikkim.

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2.

Mr. Hemant Sharma, Project Assistant,       IBSD Core

M.Sc. Microbiology, SLET & ARS NET qualified.

Email: hemant.gtk@gmail.com

Worked on : Isolation, characterisation of actinomycetes from soil & Bioassay guided purification of bioactive secondary metabolites and chitinolytic profiling of Streptomycetes.

Currently working as : Clerical staff in State Government, Sikkim state.

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3.

Miss Anjana Pradhan, M.Sc. Dissertation student (2012).

M. Sc. Biotechnology, (MSB/11/216), Amity University, Noida.

Worked on : Isolation, characterization & in-vitro antimicrobial potential of streptomycetes of Sikkim region.

Currently working as: In JICA project, Biodiversity conservation Board, Sikkim state.

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A.     Core/Internal

a.      Present:

Sl. No.

Duration

(From-To)

Title of Project/ Grant

PI/

Co-PI

Funding

Agency

Amount

1

2011 - Continuing

Screening and characterization of bioactive metabolites from actinomycetes of Sikkim Himalayas for antimicrobial potentiality.

PI

IBSD

NA

2

2011- Continuing

Isolation, characterisation and evaluation of indigenous microbial flora of Sikkim Himalayas for potential utilization as biocontrol agent.

PI

IBSD

NA

 

b. Past

Sl. No.

Duration (From-To)

Title of Project/ Grant

PI/

Co-PI

Funding Agency

Amount

1

2011-2014

Survey and field study for disease incidence of fungal blight/Anthracnose of large cardamom (Amomum subulatum Roxb.) in Sikkim.

PI

IBSD

NA

2

2011-2014

Isolation, identification & pathogenecity study of the blight pathogen (Colletotrichum gloeosporioides) of Amomum subulatum Roxb.

PI

IBSD

NA

 

B.   Extramural:

             a. Present

Sl. No.

Duration (From-To)

Title of Project/ Grant

PI/

Co-PI

Funding Agency

Amount

1

30/3/2015 –

30-09/2020

Chemical Ecology of North East Region (NER) of India: A Collaborative Programme Linking NER and Bangalore Researchers

 

PI

DBT

334.54

(Rupees 3 Crore Thirty Four Lakhs and fifty four thousand)

           b.   Past

Sl. No.

Duration (From-To)

Title of Project/ Grant

PI/

Co-PI

Funding Agency

Amount

1