Dr. Sudripta Das,Scientist-E
Area of Research
Plant Molecular Genetics & Genomics
Functional genomic analysis of medicinally important secondary metabolic pathways
The study of the chemical composition of plants has always been of great interest in biological research, in part because biochemical phenotypes largely reﬂect the developmental stage of the plant and its interactions with the environment. Investigating the mechanism of metabolism in plants by linking metabolites to genes through omics analysis—a combination of metabolome and transcriptome analyses, and the knowledge obtained from this approach would lead steadily to the successful development of functional plants. In our study, we would utilize deep transcriptomics combined with targeted metabolic profiling to identify candidate genes and transcription factors associated with targeted metabolites like aconitine and berberine. The obtained spatial and temporal datasets are useful resources for further studies involving intermediate metabolites and novel compounds.
Functional genomic analysis of aconitine and berberine production
Allele mining for stress tolerance in tea – DBT–Unit of Excellence (NERBPMC)
1. Generation and analysis of Expressed Sequence Tag (EST) from Camellia species – DBT, Govt. of India (Principal Investigator) 2006-10 (165.1 lakhs)
2. Development of integrated genetic linkage map and marker assisted selection in tea – DBT, Govt. of India (Principal Investigator) 2006-10 (145.08 lakhs)
3. Development of transgenics for blister blight resistance in tea – DBT, Govt. of India (Co-Principal Investigator) 2007-10 (24.61 lakhs)
4. Decaffeinated Tea: Through Gene Silencing – DST, Govt. of India (Principal Investigator) 2007-10 (18.08 lakhs)
5. Production of terpenoids in untransformed and transformed cell and organ cultures in tea – DBT, Govt. of India (Principal Investigator) 2007-10 (25.56 lakhs)
6. Developing production system for tea polyphenols and their condensed products –CSIR-NMITLI, Govt. of India (Principal Investigator) 2004-2009 (56.06 lakhs)
7. Characterization and enhancement of volatile flavour components and gene expression profiling in Darjeeling black tea infested by green fly and thrips – INDO-JAPAN Collaborative project, Tea Board (Principal Investigator) 2006-2009 (19.98 lakhs)
8. Abiotic and Biotic Stress Analysis for development of Stable Quality Genotypes – Tea Board 11th plan (Co-Principal Investigator) 2008-2013 (66.49 lakhs)
9. Tea Bioinformatics – Ministry of Information Technology and Communication (MICT) (Principal Investigator) 2010-2014 (97.14 lakhs)
10. Molecular documentation of Darjeeling Tea clones using DNA profiling – DBT, Govt. of India (Principal Investigator) 2010-2013 (46.23 lakhs)
11. Establishment of Institutional Biotech Hubs (IBT Hubs) by DBT under special programme for North Eastern States of India (Principal Investigator) 2010-13
12. Identification and characterization of sequence based markers for genetic improvement of tea (DBT) (Principal Investigator) 2011-14 (53.58 lakhs)
Post-Doctoral Fellow (2011-12) in Noble Research Centre, Oklahoma State University, under Prof. Ramanjulu Sunkar
IFCPAR-CEFIPRA Post-Doctoral Fellowship in 2001 and worked under Prof. David Tepfer at Lab. Rhizosphere, INRA, France
Ph.D, 1992-1998 (University of Calcutta, Kolkata) under Prof. Sumita Jha
M.Sc. Botany (University of Calcutta, Kolkata)
June 2013 – Present: Scientist-E, IBSD, Imphal
February 2013-June 2013: Scientist-E, Tocklai Tea Research Institute, Jorhat
April 2008-January 2013: Scientist-D, Tocklai Tea Research Institute, Jorhat
January 2004-March 2008: Scientist-C, Tocklai Tea Research Institute, Jorhat
1. Bornali Gohain: Designing a quality index for elite Camellia sinensis clones based on molecular and gene expression studies. 2012
2. Sushmita Gupta: Study of Transcriptome Polymorphisms and development of molecular markers associated with drought tolerance for marker assisted selection in Camellia sinensis. 2012
3. Sourabh K Das: Analysis of A Polyploid Population In Tea Using Structural/ Functional Genomic Techniques. 2013
4. Priyadarshini Bhorali: Identification of Molecular Markers Associated with Blister Bligh (Exobasidium vexans) Disease in Camellia sinensis. 2013
5. Tirthankar Bandyopadhyay: Identification of differentially expressed genes induced by infestation with Helopeltis theivora in Camellia sinensis (L.)O. Kuntze.2012
6. Raju Bharalee: Generation, analysis and identification of Expressed Sequence Tags of differentially expressed genes of Camellia sinensis (L.) O. Kuntze under abiotic stress (drought) using suppression subtractive hybridization. 2012
7. Niraj Agarwala: Study and analysis of differentially expressed genes in tea (Camellia sinensis(L).O.Kuntze)in response to Exobasidium vexans 2015
8. Ranjit Singh: Transfer and analysis of defense genes for conferring resistance against blister blight in Camellia sinensis (L).O. Kuntze. 2015
9. Parveen Ahmed: Development of Molecular Markers for Helopeltis resistance in Tea. 2014
10. Prasenjit Bhagwati: Study of genetic variability related to pigmentation and flavour formation in wild and cultivated tea germplasms. 2015
11. Neelakshi Bhattacharyya: Decaffeinated Tea: Through Gene Silencing. 2016
Awards & Fellowships
IFCPAR-CEFIPRA Post-Doctoral Fellowship in 2001
DBT-CREST Award 2011
Member of International Association for Plant Molecular Biology (ISPMB)
Member of International Association for Plant Tissue Culture & Biotechnology (IAPTC & B)
Relevant recent publications
“Construction of a genetic linkage map and mapping of drought tolerance trait in Indian beveragial tea” Sapinder Bali, Vishnu Bhat, A Mamgain, Sudripta Das, S N Raina, A K Pradhan, S K Yadava and S Goel. Mol Breeding. 2015. 35:112. [DOI 10.1007/s11032-015-0306-5].
“Enhanced resistance to blister blight in transgenic tea (Camellia sinensis [L.] O. Kuntze) by overexpression of class I chitinase gene from potato (Solanum tuberosum)”. H. Ranjit Singh, Manab Deka and Sudripta Das. Funct Integr Genomics. 2015. [DOI 10.1007/s10142-015-0436-1].
“Molecular Landscape of Helopeltis theivora Induced Transcriptome and Defense Gene Expression in Tea”. Tirthankar Bandyopadhyay, Bornali Gohain, Raju Bharalee, Sushmita Gupta, Priyadarshini Bhorali, Sourabh Kumar Das, M C. Kalita and Sudripta Das. Plant Mol Biol Rep. 2014. [DOI 10.1007/s11105-014-0811-4].
“Isolation of high quality RNA from non-cultivated Camellia species rich in anthocyanins and other polyphenols”. Prasenjit Bhagawati, Parveen Ahmed, Hijam Ranjit Singh, Manab Deka and Sudripta Das. Annals Biol. Research, 2014, 5 (5):59-63.
“Molecular Analysis of Drought Tolerance in Tea by cDNA-AFLP Based Transcript Profiling”. (2013) Sushmita Gupta, Raju Bharalee, Priyadarshini Bhorali, Sourabh K. Das, Prasenjit Bhagawati, Tirthankar Bandyopadhyay, Bornali Gohain, Niraj Agarwal, Parveen Ahmed, Sangeeta Borchetia, M. C. Kalita, A. K. Handique, Sudripta Das. Mol. Biotechnol. 53:237–248 [DOI: 10.1007/s12033-012-9517-8].
“Biochemical evaluation…….. catechin rich genotypes” (2013). S. K. Das, S. Sabhapandit, G. Ahmed and Sudripta Das. Biochem Genetics. June 2013,Volume 51,Issue 5-6, pp 358-376 [DOI: 10.1007/s10528-013-9569].
“Identification of Differentially……………Suppression Subtractive Hybridization” A Das, Sudripta Das and T K Mondal. (2012). Plant Mol Biol Rep. 30:1088–1101.
“Understanding Darjeeling Tea Flavour on a Molecular Basis” Bornali Gohain, Sangeeta Borchetia, L.P. Bhuyan, A. Rahman, K. Sakata, M. Mizutani, B. Shimizu, G. Gurusubramaniam, R. Ravindranath, M. Hazarika and Sudripta Das. Plant Mol Biol. 78: 577-597 (doi:10.1007/s11103-012-9887-0)2012.
“Identification of drought tolerant progenies in tea by gene expression analysis”. Sushmita Gupta, Raju Bharalee, Priyadarshini Bhorali, Tirthankar Bandyopadhyay, Bornali Gohain, Niraj Agarwal, Parveen Ahmed, Hemanta Saikia, Sangeeta Borchetia, MC Kalita, AK Handique and Sudripta Das. Funct Integr.Genomics (2012). 12: 543-563. [DOI: 10.1007 /s10142-012-0277-0].
“New record of nucleopolyhedroviruses in tea looper caterpillars in India”. Binu Antony, Palatty Allesh Sinu, Sudripta Das. Journal Invertebrate Pathology (2011) [DOI: 10.1016/j.jip.2011.06.007] 108(1): 63-67.