Dr. Pardeep Kumar Bhardwaj,Scientist-C (IBSD, Sikkim Centre)
Molecular mechanisms of plant adaptation in high altitude
Plant secondary metabolites and biosynthetic pathways
Chemical ecology mainly secondary metabolites and plant defense
Molecular mechanisms of adaptation and regulation of secondary metabolic pathways of important medicinal plants from Sikkim Himalaya.
Sikkim Himalaya is a part of Eastern Himalaya, which is globally known for its biodiversity. It is unique due to its close proximity to both the Tibetan Plateau and Bay of Bengal. This topography, biogeography and climate have nurtured various eco-zones in close proximity. These eco-zones have altitudinal variations ranging from humid tropical forests to temperate zones, alpine meadows and trans-himalayan cold deserts within a distance of 100 kms. Such a sharp climatic variation in different eco-zones has prompted rich biodiversity in this region. Realizing the uniqueness of niche environment, plant resources of this region offer an advantage in having greater possibilities of novel biomolecules. Therefore, Sikkim Himalaya offers tremendous research opportunities for bioprospecting novel molecules/ genes/ enzymes/ pathways/ mechanisms from the native bioresources. Earlier, we have identified genes and adaptive mechanism of plants growing in cold desert of Western Himalaya. Currently, my research interests focused on genomics, transcriptomics and metabolomics approaches to understand the molecular mechanisms of plant adaptation in high altitude of Sikkim Himalaya. I am also interested in understanding the regulation of metabolite biosynthetic pathways of important medicinal plants unique to this region and characterization of regulatory genes for metabolic engineering.
Identification of elite germplasm and molecular characterization of regulatory genes involved in ginsenoside biosynthetic pathway in Panax species from Sikkim Himalaya.
Functional genomics and biochemical analysis of xanthones/secoiridoid glycosides biosynthesis in Swertia chirayita.
Chemical Ecology of the North East Region of India: A Collaborative Programme Linking NER and Bangalore Researcher.
Ph.D. (Biotechnology), 2011, CSIR-Institute of Himalayan Bioresource Technology (IHBT), Palampur-176061, Himachal Pradesh/ Panjab University, Chandigarh-160014
M.Sc. (Biotechnology), 2001, Guru Jambheshwar University of Science & Technology, Hisar-125001, Haryana
January 2011-Present: Scientist, Institute of Bioresources and Sustainable Development (IBSD), Sikkim Centre, Gangtok-737102
April 2005-December 2010: D. Fellow, CSIR-IHBT, Palampur-176061, Himachal Pradesh
April 2002-March 2005: Project Fellow, CSIR- IHBT, Palampur-176061, Himachal Pradesh
Awards & Fellowships:
2015: Best poster award in “1st National Bioresources and Sustainable Development Summit” IBSD, Imphal
2013: Felicitation on National Science Day for publishing research work in Scientific Reports (Nature Publishing Group), CSIR-IHBT, Palampur
2005: Senior Research Fellowship by Council of Scientific and Industrial Research, Human Resource Development Ministry, Govt. of India
Life Member of Biotech Research Society of India
Life Member of Society of Biological Chemists (India)
Life Member of Him Science Congress Association
Specialized training on medicinal plants certification at CSIR-IHBT, Palampur.
NABL training on “Laboratory quality & management system and Internal auditor training” based on ISO 19011-2002 at CSIR- IHBT, Palampur.
Specialized training on “Next generation sequencing and gene expression analysis” at CSIR- IHBT, Palampur.
Science & Communication workshop organized by Wellcome Trust-DBT India Alliance at IBSD, Imphal.
- Kumar A., Sharma M., Bhardwaj P. K., Vats S. K., Singh D., Kumar S. (2016) Copper, zinc superoxide dismutase from Caragana jubata: A thermostable enzyme that functions under a broad pH and temperature window. Process Biochemistry, http://dx.doi.org/10.1016/j.procbio.2016.06.025
- Louis B., Waikhom S.D., Roy P., Bhardwaj P.K., Singh W.M., Goyari S., Sharma C.K., Talukdar N.C. (2014) Secretome weaponries of Cochliobolus lunatus interacting with potato leaf at different temperature regimes reveal a CL[xxxx]LHM – motif. BMC Genomics, 15:213. PubMed ID: 24650331
- Louis B., Waikhom S.D., Roy P., Bhardwaj P.K., Singh W.M., Sharma C.K., Talukdar N.C. (2014) Invasion of Solanum tuberosum by Aspergillus terreus: A microscopic and proteomics insight on pathogenicity. BMC Research Notes, 7:350. PubMed ID: 24917207
- Bhardwaj P.K., Deep Mala, Kumar S. (2014) 2-Cys peroxiredoxin responds to low temperature and other external cues in Caragana jubata, a plant species of cold desert of Himalaya. Molecular Biology Reports, DOI 10.1007/s11033-014-3151-4. PubMed ID: 24477582
- Louis B., Waikhom S.D., Roy P., Bhardwaj P.K., Sharma C.K., Singh W.M., Talukdar N.C. (2014) Host range dynamics of Colchliobolus lunatus: From a biocontrol agent to a severe environmental threat. Biomed Research International, 2014, Article ID 378372, doi: 10.11155/2014/378372. PubMed ID: 24987680
- Bhardwaj P.K., Kapoor R., Deep Mala, Bhagwat G., Acharya V., Singh A.K., Vats S.K., Ahuja P.S., Kumar S. (2013) Braving the attitude of altitude: Caragana jubata at work in cold desert of Himalaya. Scientific Reports (Nature Publishing Group), 3:1022: DOI:10.1038/srep01022. PubMed ID: 23289064
- Waikhom S.D., Louis B., Roy P., Singh W.M., Bhardwaj P.K., Talukdar N.C. (2013) Scanning electron microscopy of pollen structure throws light on resolving Bambusa–Dendrocalamus complex: bamboo flowering evidence. Plant Systematics and Evolution, DOI 10.1007/s00606-013-0959-7.
- Bhardwaj P.K., Kaur J, Sobti R.C., Kumar S (2012) Identification and expression analysis of CjLTI, a novel low temperature responsive gene from Caragana jubata. Molecular Biology Reports, 39: 3197–3202. PubMed ID: 21701826
- Bhardwaj P.K., Kaur J, Sobti R.C., Ahuja P.S., Kumar S (2011) Lipoxygenase gene in Caragana jubata responds to low temperature, abscisic acid, methyl jasmonate and salicylic acid. Gene, 483: 49–53. PubMed ID: 21640803
- Ghawana S, Paul A, Kumar H, Kumar A, Bhardwaj P.K., Rani A, Singh R.S, Raizada J, Singh K and Kumar S (2011) An RNA isolation system for plant tissues rich in secondary metabolites. BMC Research Notes, 4: 85. PubMed ID: 21443767
- Singh R.S., Gara R.K., Bhardwaj P.K., Malik S, Kumar R, Sharma M, Ahuja P.S., and Kumar S (2010) Expression of 3-hydroxy-3-methylglutaryl-CoA reductase, p-hydroxybenzoate-m-geranyltransferase and genes of phenylpropanoid pathway exhibits positive correlation with shikonins content in arnebia [Arnebia euchroma (Royle) Johnston]. BMC Molecular Biology, 11: 88. PubMed ID: 21092138
- Bhardwaj P.K., Ahuja P.S., Kumar S (2010) Characterization of gene expression of QM from Caragana jubata, a plant species that grows under extreme cold. Molecular Biology Reports, 37: 1003-1010. PubMed ID: 19757181
- Yogavel M, Mishra P.C., Gill J, Bhardwaj P.K., Dutt S, Kumar S, Ahuja P.S., Sharma A (2008) Structure of superoxide dismutase and implications for copper ion chelation. Acta Crystallographica Section D: Biological Crystallography, D64: 892-901. PubMed ID: 18645238
- Singh K, Raizada J, Bhardwaj P.K., Ghawana S, Rani A, Singh H, Kaul K, Kumar S (2004) 26S rRNA-based internal control gene primer pair for reverse transcription-polymerase chain reaction-based quantitative expression studies in diverse plant species. Analytical Biochemistry, 335: 330–333. PubMed ID: 15556573
Patents filed/ awarded:
- Bhardwaj P. K., Kapoor R., Bhagwat G., Ahuja P. S., Kumar S. DNA sequence in plant Caragana jubata with freeze tolerance. US Patent, US 2014/0018526 A1.
- Bhardwaj P.K., Kumar A., Kishor A., Ghawana S., Rani A et al., Method of cloning stable stress tolerant superoxide dismutase using universal primers. US Patent, US 2012/0070835 A1.
- Bhardwaj P.K., Sahoo R., Kumar S., Ahuja P.S. Superoxide dismutase gene and a method of identifying and cloning thereof. US Patent, 7888088 B2.
- Ghawana S., Singh K., Raizada J., Rani A., Bhardwaj P.K., Kumar S. A method for rapid isolation of RNA and a kit thereof. US Patent, WO 2007/113614.
- Transferred technology of the kit for RNA isolation to Bangalore Genei Pvt. Ltd. (Now a part of Merck) in 2007. (As a member of research team from CSIR-IHBT, Palampur).
|1||Mr. Bhusan GurungEmail: firstname.lastname@example.org
Problem addressing: Molecular characterization of regulatory genes involved in ginsenosides biosynthesis in Panax species.
|1||Mr. Padam Lall SharmaEmail: email@example.com
Laboratory cum field attendant
|2||Mr. Bikash RaiEmail: firstname.lastname@example.org
|1||Dr. Louis BengyellaDBT-TWAS Fellow from IBSD, Imphal (2013-2014)|
|2||Mr. Arun ShawResearch trainee from WBSU, Kolkata (2013)|
|3||Mr. Ledong LepchaResearch trainee from DST, Gangtok, Sikkim (2013)|
|4||Ms. Kriti BhagatResearch trainee from EIILM University (2014)|
|5||Mr. Pankaj TripathyResearch trainee from Sikkim University (2014)|