Dr. L. Shantikumar Singh,Scientist-B (IBSD, Sikkim Centre)
Bioprospecting for secondary metabolites from Actinomycetes, Endophytes
Biocontrol Agents, Phytochemicals against dermatophytes & opportunistic pathogens
Plant Endophyte Relation
Chemical Ecology of Endophytes, Interaction with host & their secondary metabolites
Bioprospecting of Indigenous Microbial flora including Endophytes of the Sikkim Himalayas for their potential application in Agriculture and Therapeutics
Microorganisms represent the richest repertoire of molecular and chemical diversity in nature as they underlie basic ecosystem processes. Due to their functional diversity, microorganisms have found applications in agriculture, industry, medicine and environment. Biocontrol agent which is more environments friendly involves the use of naturally occurring non pathogenic microorganism that set to reduce the activity of plant pathogens and thereby suppress diseases. The Sikkim Himalayan region being a part of the Biodiversity hot spots has enormous potential for exploration and bioprospecting of the untapped indigenous microflora which could be exploited for various useful applications. Hence tremendous opportunities in bioprospecting for novel molecules out these unique pristine habitats cannot be rule out. It’s imperative to highlight the proposition of “Renaissance in antibacterial discovery from actinomycetes”. In our view, making the uncultured from untapped habitat to pure culture is one new hope for getting new drug leads which would offer great potential to mitigate ever increasing burden of drug resistance in health care sector. Our group mainly focuses on bioprospecting of endophytes, actinomycetes & rare actinomycetes from unique pristine untapped ecological niches for their therapeutic as well as biocontrol potential towards sustainable utilization of the untapped microbial resources for the benefit of mankind.
Sub-Programme- I: Isolation, Characterisation and Evaluation of Indigenous Microbial flora of Sikkim Himalayas for potential utilization as biocontrol agent.
Sub-Programme-II: Screening and Characterization of bioactive metabolites from Actinomycetes of Sikkim Himalayas for Antimicrobial Potential.
PI of the collaborative project entitled “Chemical Ecology of the North East Region (NER) of India: A Collaborative Programme Linking NER and Bangalore Researchers”. Funding Agency: DBT, Govt. of India. Duration – 5 years.
Co-PI of the project entitled “Evaluation of role of arbuscular mycorrhizal fungi and PGPRs from diverse ecosystem of North East India for organic production of Citrus and Chili”. Funding Agency: DBT, Govt. of India (April 2012-April 2015).
Ph.D., Life Sciences (Microbial Biotechnology), 2009 (CSIR-NEIST, Jorhat, Assam; Dibrugarh University, Assam).
M.Sc., Microbiology, 2001, Bangalore University, Bengaluru.
June 2016 – Present: Scientist B, IBSD Sikkim Centre, Gangtok, Sikkim.
January 2011 – May 2016: Scientist, IBSD Sikkim Centre, Gangtok, Sikkim.
2009 – 2011: Assistant Manager R & D and Microbiologist, Research and Development Centre, APPL (Tata Tea Research), Teok, Jorhat, Assam.
2008 – 2009: Post Doctoral Research Scholar, University School of Biotechnology, Guru Gobind Singh Indraprastha University, Delhi.
2002 – 2007: JRF and SRF, DRL, DRDO, Tezpur, Assam. (Ph.D. Research work under CSIR- NEIST, Jorhat, Assam).
2001 – 2002: Lecturer, Brindavan College of UG & PG Sciences, Bangalore University, Bengaluru.
Awards & Fellowships
2009: “Group Technology Award 2009” given by DRL, DRDO, Ministry of Defence, Govt. of India, Tezpur, Assam, for the formulation and evaluation of herbal product “Herbol”, a Germicidal Floor Cleansing Emulsifier.
2002-2007: JRF and SRF awarded by Defence Research and Development Organisation (DRDO), Ministry of Defence, Govt. of India, New Delhi.
2001: “Academic Excellence Award” by Post Graduate Department of Microbiology, NFG College, Bangalore University, Bengaluru.
1992: 46th Rank in state level “First Search Talent Examination in Science” awarded by Besant Academy, Sastri Nagar, Chennai, Tamil Nadu.
1990: 2nd position in state level Quiz competition on the occasion of National Science Day awarded by Science Teachers’ Forum, Manipur.
Annual member of Indian Science Congress Association.
2002: Proficiency course in “Bioprocess Engineering” held at IISc. Bangalore, Bengaluru.
2003: Mass production of bacterial strain in 100 L Fermentor, Recovery & bio-efficacy testing of the cell mass against mosquito larvae at Vector Control Research Centre, ICMR, Puducherry.
2003: CEP course on “Recent trends in Malaria and its control” held at DRL, DRDO, Tezpur, Assam. 22nd – 26th September 2003.
2004: CEP course on “Application of Remote sensing and GIS in the management of Malaria” held at DRL, DRDO, Tezpur, Assam. 16th – 20th February 2004.
2004: CEP course on “Application of Botanicals in the management of pathogenic fungi” held at DRL, DRDO, Tezpur, Assam. 15th – 19th March 2004.
2004: CEP course on “Naturally Occurring Microbes for Control of Human Disease” held at DRL, DRDO, Tezpur, Assam. 11th – 15th October 2004.
2006: CEP course on “Molecular Approaches in Disease Diagnosis” held at DRL, DRDO, Tezpur, Assam. 17th – 21st July 2006.
2012: Workshop on “Science and Communication” sponsored by Wellcome Trust DBT India Alliance from 25th – 26th July 2012 organized by IBSD, DBT Imphal.
Laishram Shantikumar Singh, Hemant Sharma, Narayan Chandra Talukdar (2014). Production of potent antibiotic by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak lake of Manipur, India. BMC Microbiology 14:278.
Anand Nagpure, Bharti Choudhary, Shantikumar, R.K. Gupta (2014). Isolation and characterization of chitinolytic Streptomyces sp. MT7 and its antagonism towards wood-rotting fungi. Annals of Microbiology. 64 (2), 531-541.
Shantikumar Singh, S. Mazumder, T. C. Bora (2009). Optimisation of process parameters for growth and bioactive metabolite produced by salt-tolerant and alkaliphilic actinomycetes – Streptomyces tanashiensis strain A2D. Journal De Mycologie Medicale 19: 225-233.
Laishram S.K.S., Das, N. G., Bailung, B., Dube, S.N., Srivastava, R.B (2008). Evaluation of some medicinal plants of North-Eastern India for antibacterial activity: A preliminary study. J. of cell and Tissue Res. Vol. 8(1): 1285-1287.
Shantikumar Singh, Indra Baruah, T. C. Bora (2006). Actinomycetes of Loktak Habitat: Isolation & Screening for Antibacterial Activities. Biotechnology, 5 (2): 217-221.
Shantikumar Singh, Barnali Bailung, I. Baruah, T.C. Bora (2006). Evaluation of antimicrobial activity of Bacteria isolated from Loktak Lake of Manipur, India. Asian J. of Microbiol. Biotech. Env. Sc., 8 (2): 271-273.
Shantikumar Singh, T.C. Bora (2006). Antifungal spectra of activity of Streptomyces S 124 strain against plant pathogenic fungi. Indian phytopathology, 59 (3): 400.
Laishram S.K.S., Nath D.R., Bailung B., Baruah I (2006). In vitro antibacterial activity of essential oil from rhizome of Homalomena aromatica against pathogenic bacteria. J. of cell and Tissue Res. Vol. 6 (2): 849-851.
Baruah, I., Laishram S.K.S., Das, S. C., Kalita, J. (2006). Larvicidal activity of indigenous Entomopathogenic soil bacteria from Northeastern region of India. J. of cell and Tissue Res. Vol. 8 (1): 1271-1274.
Goswami, L. Shantikumar Singh (2006). Antibacterial Potential of Some Indigenous plants Against Human Pathogenic Bacteria. Environ. and Ecol. 24S (2): 403- 405.
Sharbani Mazumder, L. Shantikumar Singh and T. C. Bora. Endophytic fungi associated with medicinal plants of Gibbon Wild Life Sanctuary, India and their antagonistic properties. In: Advances in Life Sciences: Principles and Applications. Tayung, K., Barik, B.P., Mohapatra, U.B. (Eds.), Studium press LLC, Houston, USA. 2012, PP: 213 – 224. ISBN: 1-933699-69-8.
Nucleotide sequences published at NCBI GenBank database:
Paenibacillus lautus strain RRT AY-2 16 S ribosomal RNA gene, partial sequence. Accession No. DQ299946. 12- Dec- 2005.
Streptomyces sannanensis strain 118 16 S ribosomal RNA gene, partial sequence. Accession No. EU278596. 05-Dec- 2007.
Streptomyces tanashiensis strain A2D 16 S ribosomal RNA gene, partial sequence. Accession No. EU513377. 23-Feb- 2008.
Streptomyces pactum strain S124 16S ribosomal RNA gene, partial sequence. Accession No. JX570697. 10 Nov 2012.
Streptomyces exfoliatus strain RCS07 16S ribosomal RNA gene, partial sequence. Accession No. KF513157. 3rd Sept. 2013.
Streptomyces somaliensis strain RCS17 16S ribosomal RNA gene, partial sequence. Accession No. KF513158. 3rd Sept. 2013.
Kitasatospora gansuensis strain RCS37 16S ribosomal RNA gene, partial sequence. Accession No. KF513159. 3rd Sept. 2013.
Streptomyces varsoviensis strain RCSP4 16S ribosomal RNA gene, partial sequence, Accession no. KJ632657, 16 June 2014.
Streptomyces rubiginosohelvolus strain RCS120 16S ribosomal RNA gene, partial sequence. Accession No. KJ632658. 16 June 2014.
Core team member in the formulation and development of a herbal product, “Herbol” a Germicidal Floor Cleansing Emulsifier developed by DRL, DRDO Tezpur, Assam.
|Sl. No.||Name, Designation/Position & Short Introduction||Photograph|
|1.||Mr. Hemant Sharma, Project Assistant.M.Sc. Microbiology, Bangalore University, SLET Qualified.
Problem Addressing: Isolation & Bioassay guided purification of bioactive secondary metabolites and chitinolytic profiling of Streptomycetes.
|2.||Miss Subecha Rai, Junior Research Fellow.M.Sc. Microbiology, University of North Bengal.
Problem Addressing: Endophytes of Important Medicinal plants for their bioactive compounds.
|3.||Miss Okram Annie, Junior Research Fellow.M.Sc. Biochemistry, Lovely Professional University, Punjab, Batch Topper in the University.
Problem Addressing: Role of Endophytes in the biosynthesis of pharmaceutically-active metabolites.
|Previous Members (Alumni)|
|1.||Mrs. Radha Kumari Basnett, Senior Technical Assistant (2011-2012).M.Sc. Microbiology, Bangalore University.
Current position: Technical Assistant, Department of Microbiology, Sikkim University, Gangtok.
|2.||Miss Anjana Pradhan, M.Sc. Dissertation student (2012).M. Sc. Biotechnology, Amity University, Noida.
Current position: N/A
|3.||Miss Meera Ongmu Bhutia, Summer trainee student (2013).M. Sc. Medical Microbiology & M.Phil.
Current position: Ph. D. student in the Department of Microbiology, Sikkim University, Gangtok.
|4.||Miss Pooja Pradhan, Summer trainee student (2013).M.Sc. Microbiology & M.Phil.
Current position: Ph. D. student in the Department of Microbiology, Sikkim University, Gangtok.
|5.||Miss Naimia Pradhan, Summer trainee student (2014).Current position: N/A||NA|
|6.||Mr. Deepjyoti Kalita, M.Sc. Dissertation student (2014).M.Sc. Microbiology, Sikkim University.
Current position: Junior Research Fellow in Project in Centre for the Environment, IIT Guwahati.
|7.||Mr. Pritam Bardhan, M.Sc. Dissertation student (2015).M. Sc. Biotechnology, T.M. Bhagalpur University.
Current position: Ph. D. student in the Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam.